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EIF2AK1 Monoclonal antibody, PBS Only

EIF2AK1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2H1F3

Cat No : 67674-1-PBS

Print datasheet

Synonyms

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369

验证数据展示

  • WB analysis using 67674-1-Ig (same clone as 67674-1-PBS)

    WB analysis using 67674-1-Ig (same clone as 67674-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

    IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

    IHC staining of human breast cancer using 67674-1-Ig (same clone as 67674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67674-1-Ig (same clone as 67674-1-PBS)

    IF Staining of HeLa using 67674-1-Ig (same clone as 67674-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2AK1 antibody (67674-1-Ig, Clone: 2H1F3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67674-1-PBS in a different storage buffer formulation.

产品信息

67674-1-PBS targets EIF2AK1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen EIF2AK1 fusion protein Ag16970 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2-alpha kinase 1
Synonyms EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369
Calculated Molecular Weight 630 aa, 71 kDa
Observed Molecular Weight71 kDa
GenBank Accession NumberBC006524
Gene Symbol EIF2AK1
Gene ID (NCBI) 27102
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BQI3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.