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验证数据展示

  • WB analysis using 67674-1-Ig

    WB analysis using 67674-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67674-1-Ig

    IHC staining of human breast cancer using 67674-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 67674-1-Ig

    IHC staining of human breast cancer using 67674-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67674-1-Ig (EIF2AK1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67674-1-Ig

    IF Staining of HeLa using 67674-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2AK1 antibody (67674-1-Ig, Clone: 2H1F3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

EIF2AK1 Monoclonal antibody

EIF2AK1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67674-1-Ig

产品说明书

CloneNo. 2H1F3

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, HepG2 cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67674-1-Ig targets EIF2AK1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 EIF2AK1 fusion protein Ag16970 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 1
别名 EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369
计算分子量 630 aa, 71 kDa
观测分子量71 kDa
GenBank蛋白编号BC006524
基因名称 EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_2882869
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BQI3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

实验方案

Product Specific Protocols
WB protocol for EIF2AK1 antibody 67674-1-IgDownload protocol
IHC protocol for EIF2AK1 antibody 67674-1-IgDownload protocol
IF protocol for EIF2AK1 antibody 67674-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols