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验证数据展示

  • IF Staining of HeLa using CL488-67674

    IF Staining of HeLa using CL488-67674

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 EIF2AK1 antibody (CL488-67674, Clone: 2H1F3 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-67674

    FC experiment of HepG2 using CL488-67674

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated EIF2AK1 Monoclonal antibody (CL488-67674, Clone:2H1F3)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated EIF2AK1 Monoclonal antibody

EIF2AK1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67674

产品说明书

CloneNo. 2H1F3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

Heme regulated inhibitor, Heme controlled repressor, HCR, Eukaryotic translation initiation factor 2-alpha kinase 1, EC:2.7.11.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67674-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67674 targets EIF2AK1 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 EIF2AK1 fusion protein Ag16970 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 2-alpha kinase 1
别名 Heme regulated inhibitor, Heme controlled repressor, HCR, Eukaryotic translation initiation factor 2-alpha kinase 1, EC:2.7.11.1
计算分子量 630 aa, 71 kDa
观测分子量71 kDa
GenBank蛋白编号BC006524
基因名称 EIF2AK1
Gene ID (NCBI) 27102
RRIDAB_3672958
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BQI3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF2AK1 antibody CL488-67674Download protocol
Standard Protocols
Click here to view our Standard Protocols