CoraLite® Plus 488-conjugated EIF2AK1 Monoclonal antibody
EIF2AK1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2H1F3
验证数据展示
经过测试的应用
Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67674-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67674 targets EIF2AK1 in IF applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | EIF2AK1 fusion protein Ag16970 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | eukaryotic translation initiation factor 2-alpha kinase 1 |
Synonyms | EIF2AK1, HCR, Heme controlled repressor, Heme regulated inhibitor, HRI, KIAA1369 |
Calculated Molecular Weight | 630 aa, 71 kDa |
Observed Molecular Weight | 71 kDa |
GenBank Accession Number | BC006524 |
Gene Symbol | EIF2AK1 |
Gene ID (NCBI) | 27102 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q9BQI3 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic initiation factor 2a (eIF2a), which is a critical component in the formation of the translation initiation ternary complex (eIF2a/GTP/methionyl transfer RNA), plays a key role in the ER stress response. EIF2AK1, which is activated by heme deficiency and other stimuli, is a major kinase that phosphorylates EIF2-alpha. Phosphorylation of the alpha subunit of EIF2 downregulates protein synthesis in response to various stress conditions. EIF2AK1 has some isoforms with 630aa, 577aa and 422aa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 EIF2AK1 antibody CL488-67674 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |