验证数据展示
经过测试的应用
| Positive WB detected in | MCF7 cells, HeLa cells, K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse brain tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18010-1-AP targets EIF2B1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | EIF2B1 fusion protein Ag12509 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa |
| 别名 | Translation initiation factor eIF2B subunit alpha, EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha |
| 计算分子量 | 305 aa, 34 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC103763 |
| 基因名称 | EIF2B1 |
| Gene ID (NCBI) | 1967 |
| RRID | AB_2261996 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14232 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF2B1 antibody 18010-1-AP | Download protocol |
| IHC protocol for EIF2B1 antibody 18010-1-AP | Download protocol |
| IF protocol for EIF2B1 antibody 18010-1-AP | Download protocol |
| IP protocol for EIF2B1 antibody 18010-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Microbiol Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association. | ||
Elife Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response. | ||
Elife The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes. | ||
FASEB J Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation. |
