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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated EIF2B1 Polyclonal antibody

EIF2B1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-18010

Print datasheet

Synonyms

Translation initiation factor eIF2B subunit alpha, EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha

验证数据展示

  • IF Staining of HeLa using CL488-18010

    IF Staining of HeLa using CL488-18010

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 EIF2B1 antibody (CL488-18010) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-18010

    FC experiment of HeLa using CL488-18010

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Eif2b1 Polyclonal Antibody (CL488-18010)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 18010-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-18010 targets EIF2B1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen EIF2B1 fusion protein Ag12509 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
Synonyms Translation initiation factor eIF2B subunit alpha, EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha
Calculated Molecular Weight 305 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC103763
Gene Symbol EIF2B1
Gene ID (NCBI) 1967
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14232
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF2B1 antibody CL488-18010Download protocol
FC protocol for CL Plus 488 EIF2B1 antibody CL488-18010Download protocol
Standard Protocols
Click here to view our Standard Protocols