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EIF2S1 Recombinant antibody, PBS Only

EIF2S1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230245A12

Cat no : 82936-1-PBS

Print datasheet

Synonyms

EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A, eIF 2 alpha

验证数据展示

  • WB analysis using 82936-1-RR (same clone as 82936-1-PBS)

    WB analysis using 82936-1-RR (same clone as 82936-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82936-1-RR (same clone as 82936-1-PBS)

    IF Staining of HepG2 using 82936-1-RR (same clone as 82936-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF2S1 antibody (82936-1-RR, Clone: 230245A12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

  • WB analysis using 82936-1-RR (same clone as 82936-1-PBS)

    WB analysis using 82936-1-RR (same clone as 82936-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82936-1-RR (EIF2S1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82936-1-RR (same clone as 82936-1-PBS)

    FC experiment of HeLa using 82936-1-RR (same clone as 82936-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human EIF2S1 (82936-1-RR, Clone:230245A12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 82936-1-RR (same clone as 82936-1-PBS)

    IP experiment of HepG2 using 82936-1-RR (same clone as 82936-1-PBS)

    IP result of anti-EIF2S1 (IP:82936-1-RR, 4ug; Detection:82936-1-RR 1:3000) with HepG2 cells lysate 1470 ug. This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82936-1-RR (same clone as 82936-1-PBS)

    IHC staining of mouse brain using 82936-1-RR (same clone as 82936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82936-1-RR (EIF2S1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 82936-1-RR (same clone as 82936-1-PBS)

    IHC staining of mouse brain using 82936-1-RR (same clone as 82936-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82936-1-RR (EIF2S1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82936-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82936-1-RR

    Affinity and Kinetic Characterization of 82936-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82936-1-RR against Human EIF2S1 were performed. The affinity constant is below 1 pM.

产品信息

82936-1-PBS targets EIF2S1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity rat, mouse, human
Immunogen EIF2S1 fusion protein Ag1645 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
Synonyms EIF2S1/EIF2A, EIF2, EIF 2alpha, EIF 2A, eIF 2 alpha
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC002513
Gene Symbol EIF2S1
Gene ID (NCBI) 1965
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05198
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF2S1 is one subunit of the translation initiation factor EIF2, which catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2A (Gene ID: 83939) and EIF2S1 (Gene ID: 1965) share the EIF2A symbol/alias in common. EIF2S1 is the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40S ribosomal subunit, the EIF2A protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP.