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验证数据展示

  • WB analysis using 82512-1-RR (same clone as 82512-1-PBS)

    WB analysis using 82512-1-RR (same clone as 82512-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • WB analysis of rat liver using 82512-1-RR (same clone as 82512-1-PBS)

    WB analysis of rat liver using 82512-1-RR (same clone as 82512-1-PBS)

    rat liver tissue were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:30300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 82512-1-RR (same clone as 82512-1-PBS)

    WB analysis of HEK-293 using 82512-1-RR (same clone as 82512-1-PBS)

    WB result of EIF3D antibody (82512-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF3D transfected HEK-293 cells. This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • IP experiment of A549 using 82512-1-RR (same clone as 82512-1-PBS)

    IP experiment of A549 using 82512-1-RR (same clone as 82512-1-PBS)

    IP result of anti-EIF3D (IP:82512-1-RR, 4ug; Detection:82512-1-RR 1:5000) with A549 cells lysate 920 ug. This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 82512-1-RR (same clone as 82512-1-PBS)

    IHC staining of human breast cancer using 82512-1-RR (same clone as 82512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3D antibody included
  • IHC staining of human breast cancer using 82512-1-RR (same clone as 82512-1-PBS)

    IHC staining of human breast cancer using 82512-1-RR (same clone as 82512-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF3D antibody included
  • IF Staining of U2OS using 82512-1-RR (same clone as 82512-1-PBS)

    IF Staining of U2OS using 82512-1-RR (same clone as 82512-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using EIF3D antibody (82512-1-RR, Clone: 3O8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82512-1-RR (same clone as 82512-1-PBS)

    IF Staining of U2OS using 82512-1-RR (same clone as 82512-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using EIF3D antibody (82512-1-RR, Clone: 3O8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82512-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82512-1-RR

    Affinity and Kinetic Characterization of 82512-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82512-1-RR against Human EIF3D were performed. The affinity constant is 5.83 nM.

  • Featured Product
  • KD/KO验证

EIF3D Recombinant antibody, PBS Only

EIF3D Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 82512-1-PBS

产品说明书

CloneNo. 3O8

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66, eIF 3 zeta

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EIF3D Ready-To-Use IHC Kit. EIF3D primary antibody included.

产品信息

82512-1-PBS targets EIF3D in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 EIF3D fusion protein Ag0268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit D
别名 eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66, eIF 3 zeta
计算分子量 66 kDa
观测分子量65 kDa
GenBank蛋白编号BC000328
基因名称 EIF3D
Gene ID (NCBI) 8664
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15371
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.