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  • Featured Product
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EIF3D Recombinant antibody

EIF3D Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3O8

Cat no : 82512-1-RR

Print datasheet

Synonyms

eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66, eIF 3 zeta

验证数据展示

  • WB analysis using 82512-1-RR

    WB analysis using 82512-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat liver using 82512-1-RR

    WB analysis of rat liver using 82512-1-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 82512-1-RR (EIF3D antibody) at dilution of 1:30300 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82512-1-RR

    WB analysis of HEK-293 using 82512-1-RR

    WB result of EIF3D antibody (82512-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF3D transfected HEK-293 cells.

  • IP experiment of A549 using 82512-1-RR

    IP experiment of A549 using 82512-1-RR

    IP result of anti-EIF3D (IP:82512-1-RR, 4ug; Detection:82512-1-RR 1:5000) with A549 cells lysate 920 ug.

  • IHC staining of human breast cancer using 82512-1-RR

    IHC staining of human breast cancer using 82512-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with EIF3D antibody included
  • IHC staining of human breast cancer using 82512-1-RR

    IHC staining of human breast cancer using 82512-1-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 82512-1-RR (EIF3D antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with EIF3D antibody included
  • IF Staining of U2OS using 82512-1-RR

    IF Staining of U2OS using 82512-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using EIF3D antibody (82512-1-RR, Clone: 3O8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of U2OS using 82512-1-RR

    IF Staining of U2OS using 82512-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using EIF3D antibody (82512-1-RR, Clone: 3O8 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 82512-1-RR

    Affinity and Kinetic Characterization of 82512-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82512-1-RR against Human EIF3D were performed. The affinity constant is 5.83 nM.

经过测试的应用

Positive WB detected inHeLa cells, rat liver tissue, HEK-293 cells, HepG2 cells, A549 cells, A431 cells, Jurkat cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy EIF3D Ready-To-Use IHC Kit. EIF3D primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82512-1-RR targets EIF3D in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, rat
Immunogen EIF3D fusion protein Ag0268 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit D
Synonyms eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66, eIF 3 zeta
Calculated Molecular Weight 66 kDa
Observed Molecular Weight65 kDa
GenBank Accession NumberBC000328
Gene Symbol EIF3D
Gene ID (NCBI) 8664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15371
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.

实验方案

Product Specific Protocols
WB protocol for EIF3D antibody 82512-1-RRDownload protocol
IHC protocol for EIF3D antibody 82512-1-RRDownload protocol
IF protocol for EIF3D antibody 82512-1-RRDownload protocol
IP protocol for EIF3D antibody 82512-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols