验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy EIF3D Ready-To-Use IHC Kit. EIF3D primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82512-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82512 targets EIF3D in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | human, rat |
| 免疫原 | EIF3D fusion protein Ag0268 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 3, subunit D |
| 别名 | eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66, eIF 3 zeta |
| 计算分子量 | 66 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC000328 |
| 基因名称 | EIF3D |
| Gene ID (NCBI) | 8664 |
| RRID | AB_3673067 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | O15371 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 EIF3D antibody CL488-82512 | Download protocol |
| FC protocol for CL Plus 488 EIF3D antibody CL488-82512 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |