CoraLite® Plus 488-conjugated EIF3D Recombinant antibody
EIF3D Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3O8
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy EIF3D Ready-To-Use IHC Kit. EIF3D primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 82512-1-PBS
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-82512 targets EIF3D in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, rat |
| Immunogen | EIF3D fusion protein Ag0268 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 3, subunit D |
| Synonyms | eIF-3-zeta, EIF3S7, eIF3 zeta, eIF3 p66, eIF 3 zeta |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC000328 |
| Gene Symbol | EIF3D |
| Gene ID (NCBI) | 8664 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O15371 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian translation initiation factor 3 (eIF3), is a multiprotein complex of ~600 kDa that binds to the 40 S ribosome and promotes the binding of methionyl-tRNAi and mRNA. The EIF3S7(p66) is the major RNA binding subunit in this complex. Human eIF3-p66 shares 64% sequence identity with a hypothetical Caenorhabditis elegans protein, presumably the p66 homolog. Deletion analyses of recombinant derivatives of eIF3-p66 show that the RNA-binding domain lies within an N-terminal 71-amino acid region rich in lysine and arginine.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 EIF3D antibody CL488-82512 | Download protocol |
| FC protocol for CL Plus 488 EIF3D antibody CL488-82512 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |