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验证数据展示

  • WB analysis using 67719-1-Ig

    WB analysis using 67719-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67719-1-Ig (EIF3L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 67719-1-Ig

    IHC staining of mouse pancreas using 67719-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67719-1-Ig (EIF3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 67719-1-Ig

    IHC staining of mouse pancreas using 67719-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67719-1-Ig (EIF3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 67719-1-Ig

    IF Staining of mouse pancreas using 67719-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse pancreas using 67719-1-Ig

    IF Staining of mouse pancreas using 67719-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • FC experiment of HeLa using 67719-1-Ig

    FC experiment of HeLa using 67719-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EIF3L (67719-1-Ig, Clone:1B11A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 67719-1-Ig

    IF Staining of HeLa using 67719-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

EIF3L Monoclonal antibody

EIF3L Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA
Cat No. 67719-1-Ig

产品说明书

CloneNo. 1B11A10

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

别名

Eukaryotic translation initiation factor 3 subunit E-interacting protein, Eukaryotic translation initiation factor 3 subunit 6-interacting protein, EIF3S6IP, EIF3S11, EIF3EIP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells, PC-12 cells, 4T1 cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67719-1-Ig targets EIF3L in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EIF3L fusion protein Ag26680 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit L
别名 Eukaryotic translation initiation factor 3 subunit E-interacting protein, Eukaryotic translation initiation factor 3 subunit 6-interacting protein, EIF3S6IP, EIF3S11, EIF3EIP
计算分子量 67 kDa
观测分子量67 kDa
GenBank蛋白编号BC007510
基因名称 EIF3L
Gene ID (NCBI) 51386
RRIDAB_2882907
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y262
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EIF3L antibody 67719-1-IgDownload protocol
IHC protocol for EIF3L antibody 67719-1-IgDownload protocol
IF protocol for EIF3L antibody 67719-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols