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验证数据展示

  • FC experiment of HeLa using CL647-67719

    FC experiment of HeLa using CL647-67719

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 647 Anti-Human EIF3L (CL647-67719, Clone:1B11A10) (red), or 0.8 ug CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 647-conjugated EIF3L Monoclonal antibody

EIF3L Monoclonal Antibody for FC (Intra)
Cat No. CL647-67719

产品说明书

CloneNo. 1B11A10

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

FC (Intra)

别名

EIF3EIP, EIF3L, EIF3S11, EIF3S6IP, HSPC021, HSPC025, MSTP005

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67719-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-67719 targets EIF3L in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse, Rat
免疫原 EIF3L fusion protein Ag26680 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit L
别名 EIF3EIP, EIF3L, EIF3S11, EIF3S6IP, HSPC021, HSPC025, MSTP005
计算分子量 67 kDa
观测分子量67 kDa
GenBank蛋白编号BC007510
基因名称 EIF3L
Gene ID (NCBI) 51386
RRIDAB_3084826
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y262
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 EIF3L antibody CL647-67719Download protocol
Standard Protocols
Click here to view our Standard Protocols