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CoraLite® Plus 488-conjugated EIF3M Monoclonal antibody

EIF3M Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A12B2

Cat no : CL488-66025

Print datasheet

Synonyms

B5, EIF3M, Fetal lung protein B5, FLJ29030, GA17, hfl B5, HFLB5, PCID1, PNAS 125

验证数据展示

  • IF Staining of MCF-7 using CL488-66025

    IF Staining of MCF-7 using CL488-66025

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 EIF3M antibody (CL488-66025, Clone: 2A12B2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3M Ready-To-Use IHC Kit. EIF3M primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66025-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66025 targets EIF3M in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen EIF3M fusion protein Ag17965 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 3, subunit M
Synonyms B5, EIF3M, Fetal lung protein B5, FLJ29030, GA17, hfl B5, HFLB5, PCID1, PNAS 125
Calculated Molecular Weight 374 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC019103
Gene Symbol EIF3M
Gene ID (NCBI) 10480
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7L2H7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF3M antibody CL488-66025Download protocol
Standard Protocols
Click here to view our Standard Protocols