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验证数据展示

  • IF Staining of MCF-7 using CL488-66025

    IF Staining of MCF-7 using CL488-66025

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 EIF3M antibody (CL488-66025, Clone: 2A12B2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated EIF3M Monoclonal antibody

EIF3M Monoclonal Antibody for IF/ICC
Cat No. CL488-66025

产品说明书

CloneNo. 2A12B2

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC

别名

B5, EIF3M, Fetal lung protein B5, FLJ29030, GA17, hfl B5, HFLB5, PCID1, PNAS 125

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3M Ready-To-Use IHC Kit. EIF3M primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66025-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66025 targets EIF3M in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 EIF3M fusion protein Ag17965 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 3, subunit M
别名 B5, EIF3M, Fetal lung protein B5, FLJ29030, GA17, hfl B5, HFLB5, PCID1, PNAS 125
计算分子量 374 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC019103
基因名称 EIF3M
Gene ID (NCBI) 10480
RRIDAB_2934445
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ7L2H7
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF3M gene encodes eukaryotic translation initiation factor (eIF) subunit M, also called GA17 or PCID1. eIF-3 complex is required for several steps in the initiation of protein synthesis, and recent studies indicate that regulation of oncogene expression and neoplastic transformation are controlled by eIF subunits. The most uncharacterized non-core subunit EIF3M was confirmed to be highly expressed in human cancer cell lines and colon cancer patient tissues and mediate regulation of tumorigenesis-related genes in human colon cancer.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF3M antibody CL488-66025Download protocol
Standard Protocols
Click here to view our Standard Protocols