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Host
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • WB analysis using 67799-1-Ig (same clone as 67799-1-PBS)

    WB analysis using 67799-1-Ig (same clone as 67799-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67799-1-Ig (EIF4A2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

    IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

    IF Staining of HEK-293 using 67799-1-Ig (same clone as 67799-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67799-1-PBS in a different storage buffer formulation.

EIF4A2 Monoclonal antibody, PBS Only

EIF4A2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67799-1-PBS

产品说明书

CloneNo. 2D11E11

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

EIF4A, eIF 4A II, EC:3.6.4.13, DDX2B, BM 010

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EIF4A2 Ready-To-Use IHC Kit. EIF4A2 primary antibody included.

产品信息

67799-1-PBS targets EIF4A2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EIF4A2 fusion protein Ag9085 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4A, isoform 2
别名 EIF4A, eIF 4A II, EC:3.6.4.13, DDX2B, BM 010
计算分子量 408 aa, 46 kDa
观测分子量46 kDa
GenBank蛋白编号BC012547
基因名称 EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2918563
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14240
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.