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验证数据展示

  • WB analysis using 67799-1-Ig

    WB analysis using 67799-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67799-1-Ig (EIF4A2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 67799-1-Ig

    IF Staining of HEK-293 using 67799-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of HEK-293 using 67799-1-Ig

    IF Staining of HEK-293 using 67799-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using EIF4A2 antibody (67799-1-Ig, Clone: 2D11E11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

EIF4A2 Monoclonal antibody

EIF4A2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67799-1-Ig
CloneNo. 2D11E11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

EIF4A, eIF 4A II, EC:3.6.4.13, DDX2B, BM 010

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4A2 Ready-To-Use IHC Kit. EIF4A2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67799-1-Ig targets EIF4A2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EIF4A2 fusion protein Ag9085 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4A, isoform 2
Synonyms EIF4A, eIF 4A II, EC:3.6.4.13, DDX2B, BM 010
Calculated Molecular Weight 408 aa, 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC012547
Gene Symbol EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2918563
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14240
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

实验方案

Product Specific Protocols
WB protocol for EIF4A2 antibody 67799-1-IgDownload protocol
IF protocol for EIF4A2 antibody 67799-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols