验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
| Positive FC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy EIF4A2 Ready-To-Use IHC Kit. EIF4A2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67799-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67799 targets EIF4A2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Rat, Mouse samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | Human, Rat, Mouse |
| 免疫原 | EIF4A2 fusion protein Ag9085 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | eukaryotic translation initiation factor 4A, isoform 2 |
| 别名 | BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F |
| 计算分子量 | 408 aa, 46 kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | BC012547 |
| 基因名称 | EIF4A2 |
| Gene ID (NCBI) | 1974 |
| RRID | AB_2920186 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14240 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 EIF4A2 antibody CL594-67799 | Download protocol |
| FC protocol for CL594 EIF4A2 antibody CL594-67799 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |