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CoraLite®594-conjugated EIF4A2 Monoclonal antibody

EIF4A2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Rat, Mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2D11E11

Cat no : CL594-67799

Print datasheet

Synonyms

BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F

验证数据展示

  • IF Staining of HEK-293 using CL594-67799

    IF Staining of HEK-293 using CL594-67799

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite®594 EIF4A2 antibody (CL594-67799, Clone: 2D11E11 ) at dilution of 1:200.

  • FC experiment of HEK-293 using CL594-67799

    FC experiment of HEK-293 using CL594-67799

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human EIF4A2 (CL594-67799, Clone:2D11E11) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHEK-293 cells
Positive FC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4A2 Ready-To-Use IHC Kit. EIF4A2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67799-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67799 targets EIF4A2 in IF/ICC, FC (Intra) applications and shows reactivity with Human, Rat, Mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human, Rat, Mouse
Immunogen EIF4A2 fusion protein Ag9085 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4A, isoform 2
Synonyms BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F
Calculated Molecular Weight 408 aa, 46 kDa
Observed Molecular Weight46 kDa
GenBank Accession NumberBC012547
Gene Symbol EIF4A2
Gene ID (NCBI) 1974
RRIDAB_2920186
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14240
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.

实验方案

Product Specific Protocols
IF protocol for CL594 EIF4A2 antibody CL594-67799Download protocol
FC protocol for CL594 EIF4A2 antibody CL594-67799Download protocol
Standard Protocols
Click here to view our Standard Protocols