验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HEK-293 cells, HeLa cells |
| Positive FC detected in | HEK-293 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy EIF4A2 Ready-To-Use IHC Kit. EIF4A2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67799-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-67799 targets EIF4A2 in FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | EIF4A2 fusion protein Ag9085 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 4A, isoform 2 |
| Synonyms | BM 010, DDX2B, eIF 4A II, EIF4A, eIF4A II, EIF4A2, EIF4F |
| Calculated Molecular Weight | 408 aa, 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC012547 |
| Gene Symbol | EIF4A2 |
| Gene ID (NCBI) | 1974 |
| RRID | AB_2935087 |
| Conjugate | CoraLite® Plus 647 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14240 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Eukaryotic initiation factor 4A (EIF4A) has an essential role in the binding of mRNA to the 43S preinitiation complex when protein synthesis begins, as EIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. EIF4A2 is a ATP-dependent RNA helicase, which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 EIF4A2 antibody CL647-67799 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
