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验证数据展示

  • IF Staining of HepG2 using CL488-67740

    IF Staining of HepG2 using CL488-67740

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 EIF4A3 antibody (CL488-67740, Clone: 1G6H9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated EIF4A3 Monoclonal antibody

EIF4A3 Monoclonal Antibody for IF/ICC
Cat No. CL488-67740

产品说明书

CloneNo. 1G6H9

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse

应用

IF/ICC

别名

DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF4A3 Ready-To-Use IHC Kit. EIF4A3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67740-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67740 targets EIF4A3 in IF/ICC applications and shows reactivity with Human, mouse samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, mouse
免疫原 EIF4A3 fusion protein Ag11130 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 4A, isoform 3
别名 DDX48, DEAD box protein 48, eIF 4A III, eIF4A III, EIF4A3, eIF4AIII, hNMP 265, hNMP265, KIAA0111, Nuclear matrix protein 265, NUK 34
计算分子量 47 kDa
观测分子量47 kDa
GenBank蛋白编号BC003662
基因名称 EIF4A3
Gene ID (NCBI) 9775
RRIDAB_2919534
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP38919
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF4A3 antibody CL488-67740Download protocol
Standard Protocols
Click here to view our Standard Protocols