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  • Featured Product
  • KD/KO Validated

EIF4E Monoclonal antibody, PBS Only

EIF4E Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

3C6B9

Cat no : 66655-1-PBS

Print datasheet

Synonyms

eIF-4F 25 kDa subunit, EIF4F, EIF4EL1, eIF-4E, eIF 4E

验证数据展示

  • WB analysis using 66655-1-Ig (same clone as 66655-1-PBS)

    WB analysis using 66655-1-Ig (same clone as 66655-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66655-1-Ig (EIF4E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66655-1-Ig (same clone as 66655-1-PBS)

    IHC staining of human breast cancer using 66655-1-Ig (same clone as 66655-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66655-1-Ig (EIF4E antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EIF4E antibody included
  • WB analysis of HEK-293 using 66655-1-Ig (same clone as 66655-1-PBS)

    WB analysis of HEK-293 using 66655-1-Ig (same clone as 66655-1-PBS)

    WB result of EIF4E antibody (66655-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF4E transfected HEK-293 cells. This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66655-1-Ig (same clone as 66655-1-PBS)

    FC experiment of HepG2 using 66655-1-Ig (same clone as 66655-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human EIF4E (66655-1-Ig, Clone:3C6B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

  • WB analysis using 66655-1-Ig (same clone as 66655-1-PBS)

    WB analysis using 66655-1-Ig (same clone as 66655-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66655-1-Ig (EIF4E antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66655-1-Ig (same clone as 66655-1-PBS)

    IF Staining of HepG2 using 66655-1-Ig (same clone as 66655-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EIF4E antibody (66655-1-Ig, Clone: 3C6B9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66655-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EIF4E Ready-To-Use IHC Kit. EIF4E primary antibody included.

产品信息

66655-1-PBS targets EIF4E in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EIF4E fusion protein Ag27191 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 4E
Synonyms eIF-4F 25 kDa subunit, EIF4F, EIF4EL1, eIF-4E, eIF 4E
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 26-29 kDa
GenBank Accession NumberBC012611
Gene Symbol EIF4E
Gene ID (NCBI) 1977
RRIDAB_2882012
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06730
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic translation initiation factor 4E, also known as eIF4E, is a protein that in humans is encoded by the EIF4E gene. eIF4E is the mRNA cap-binding protein, known as a general initiation factor allowing for mRNA-ribosome interaction and cap-dependent translation in eukaryotic cells. eIF4E is a polypeptide that exists as both a free form and as part of the eIF4F pre-initiation complex. Regulation of eIF4E may be achieved via three distinct mechanisms: transcription, phosphorylation, and inhibitory proteins.