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验证数据展示

  • WB analysis using 67907-1-Ig (same clone as 67907-1-PBS)

    WB analysis using 67907-1-Ig (same clone as 67907-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67907-1-Ig (EIF5A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67907-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67907-1-Ig (same clone as 67907-1-PBS)

    IHC staining of human lung cancer using 67907-1-Ig (same clone as 67907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67907-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67907-1-Ig (same clone as 67907-1-PBS)

    IHC staining of human lung cancer using 67907-1-Ig (same clone as 67907-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67907-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67907-1-Ig (same clone as 67907-1-PBS)

    IF Staining of HepG2 using 67907-1-Ig (same clone as 67907-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EIF5A2 antibody (67907-1-Ig, Clone: 1F9B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67907-1-PBS in a different storage buffer formulation.

EIF5A2 Monoclonal antibody, PBS Only

EIF5A2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67907-1-PBS

产品说明书

CloneNo. 1F9B2

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

eIF 5A 2, EIF 5A2, EIF5A2, eIF5AII

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67907-1-PBS targets EIF5A2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, rat
免疫原 EIF5A2 fusion protein Ag10852 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 5A2
别名 eIF 5A 2, EIF 5A2, EIF5A2, eIF5AII
计算分子量 153 aa, 17 kDa
观测分子量20 kDa
GenBank蛋白编号BC036072
基因名称 EIF5A2
Gene ID (NCBI) 56648
RRIDAB_2918663
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9GZV4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Eukaryotic translation initiation factor 5A (EIF5A), a conserved lysine to hypusine, is strictly indispensable for the survival of eukaryotic cells. There are two EIF5A isoforms in humans: EIF5A1 and EIF5A2. EIF5A2 has been reported to be overexpressed in gallbladder cancer, upper urinary tract urothelial carcinoma, gastric cancer, prostate cancer, nasopharyngeal carcinoma, and cervical cancer. EIF5A2 upregulation in cancer tissues is associated with poor prognosis in these patients.