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验证数据展示

  • WB analysis using 67907-1-Ig

    WB analysis using 67907-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67907-1-Ig (EIF5A2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human lung cancer using 67907-1-Ig

    IHC staining of human lung cancer using 67907-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 67907-1-Ig

    IHC staining of human lung cancer using 67907-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67907-1-Ig (EIF5A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67907-1-Ig

    IF Staining of HepG2 using 67907-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using EIF5A2 antibody (67907-1-Ig, Clone: 1F9B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

EIF5A2 Monoclonal antibody

EIF5A2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67907-1-Ig

产品说明书

CloneNo. 1F9B2

宿主/亚型

Mouse / IgG1

种属反应性

Human, rat

应用

WB, IHC, IF/ICC, ELISA

别名

eIF 5A 2, EIF 5A2, EIF5A2, eIF5AII

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected inhuman lung cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67907-1-Ig targets EIF5A2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, rat
免疫原 EIF5A2 fusion protein Ag10852 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 5A2
别名 eIF 5A 2, EIF 5A2, EIF5A2, eIF5AII
计算分子量 153 aa, 17 kDa
观测分子量20 kDa
GenBank蛋白编号BC036072
基因名称 EIF5A2
Gene ID (NCBI) 56648
RRIDAB_2918663
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9GZV4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 5A (EIF5A), a conserved lysine to hypusine, is strictly indispensable for the survival of eukaryotic cells. There are two EIF5A isoforms in humans: EIF5A1 and EIF5A2. EIF5A2 has been reported to be overexpressed in gallbladder cancer, upper urinary tract urothelial carcinoma, gastric cancer, prostate cancer, nasopharyngeal carcinoma, and cervical cancer. EIF5A2 upregulation in cancer tissues is associated with poor prognosis in these patients.

实验方案

Product Specific Protocols
WB protocol for EIF5A2 antibody 67907-1-IgDownload protocol
IHC protocol for EIF5A2 antibody 67907-1-IgDownload protocol
IF protocol for EIF5A2 antibody 67907-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols