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CoraLite® Plus 488-conjugated EIF5A2 Monoclonal antibody

EIF5A2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F9B2

Cat no : CL488-67907

Print datasheet

Synonyms

EIF5A1/EIF5A2, eIF5AII, eIF-5A-2, eIF-5A2, EIF 5A2

验证数据展示

  • FC experiment of HepG2 using CL488-67907

    FC experiment of HepG2 using CL488-67907

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EIF5A2 (CL488-67907, Clone:1F9B2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL488-67907

    IF Staining of HepG2 using CL488-67907

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 EIF5A2 antibody (CL488-67907, Clone: 1F9B2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67907-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67907 targets EIF5A2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, rat
Immunogen EIF5A2 fusion protein Ag10852 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 5A2
Synonyms EIF5A1/EIF5A2, eIF5AII, eIF-5A-2, eIF-5A2, EIF 5A2
Calculated Molecular Weight 153 aa, 17 kDa
Observed Molecular Weight20 kDa
GenBank Accession NumberBC036072
Gene Symbol EIF5A2
Gene ID (NCBI) 56648
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9GZV4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 5A (EIF5A), a conserved lysine to hypusine, is strictly indispensable for the survival of eukaryotic cells. There are two EIF5A isoforms in humans: EIF5A1 and EIF5A2. EIF5A2 has been reported to be overexpressed in gallbladder cancer, upper urinary tract urothelial carcinoma, gastric cancer, prostate cancer, nasopharyngeal carcinoma, and cervical cancer. EIF5A2 upregulation in cancer tissues is associated with poor prognosis in these patients.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF5A2 antibody CL488-67907Download protocol
FC protocol for CL Plus 488 EIF5A2 antibody CL488-67907Download protocol
Standard Protocols
Click here to view our Standard Protocols