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验证数据展示

  • FC experiment of HepG2 using CL488-67907

    FC experiment of HepG2 using CL488-67907

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human EIF5A2 (CL488-67907, Clone:1F9B2) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of HepG2 using CL488-67907

    IF Staining of HepG2 using CL488-67907

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 EIF5A2 antibody (CL488-67907, Clone: 1F9B2 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated EIF5A2 Monoclonal antibody

EIF5A2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67907

产品说明书

CloneNo. 1F9B2

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

IF/ICC, FC (Intra)

别名

EIF5A1/EIF5A2, eIF5AII, eIF-5A-2, eIF-5A2, EIF 5A2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67907-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67907 targets EIF5A2 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat
免疫原 EIF5A2 fusion protein Ag10852 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 5A2
别名 EIF5A1/EIF5A2, eIF5AII, eIF-5A-2, eIF-5A2, EIF 5A2
计算分子量 153 aa, 17 kDa
观测分子量20 kDa
GenBank蛋白编号BC036072
基因名称 EIF5A2
Gene ID (NCBI) 56648
RRIDAB_2934587
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9GZV4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Eukaryotic translation initiation factor 5A (EIF5A), a conserved lysine to hypusine, is strictly indispensable for the survival of eukaryotic cells. There are two EIF5A isoforms in humans: EIF5A1 and EIF5A2. EIF5A2 has been reported to be overexpressed in gallbladder cancer, upper urinary tract urothelial carcinoma, gastric cancer, prostate cancer, nasopharyngeal carcinoma, and cervical cancer. EIF5A2 upregulation in cancer tissues is associated with poor prognosis in these patients.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EIF5A2 antibody CL488-67907Download protocol
FC protocol for CL Plus 488 EIF5A2 antibody CL488-67907Download protocol
Standard Protocols
Click here to view our Standard Protocols