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验证数据展示

  • WB analysis of mouse brain using 13527-1-AP (same clone as 13527-1-PBS)

    WB analysis of mouse brain using 13527-1-AP (same clone as 13527-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13527-1-AP (EIF5B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 13527-1-AP (same clone as 13527-1-PBS)

    IHC staining of human gliomas using 13527-1-AP (same clone as 13527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 13527-1-AP (same clone as 13527-1-PBS)

    IHC staining of human gliomas using 13527-1-AP (same clone as 13527-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 13527-1-AP (EIF5B antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 13527-1-AP (same clone as 13527-1-PBS)

    IF Staining of MCF-7 using 13527-1-AP (same clone as 13527-1-PBS)

    Immunofluorescent analysis of MCF-7 cells, using EIF5B antibody 13527-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 13527-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EIF5B Polyclonal antibody, PBS Only

EIF5B Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13527-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

13527-1-PBS targets EIF5B in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EIF5B fusion protein Ag4404 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 5B
别名
计算分子量 1220 aa, 139 kDa
观测分子量 175 kDa
GenBank蛋白编号BC032639
基因名称 EIF5B
Gene ID (NCBI) 9669
RRIDAB_2096200
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60841
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Translation initiation requires the delivery of the initiator methionine tRNA to the 40S ribosomal subunit. The initiator methionine tRNA is delivered by the heterotrimeric complex EIF2 in a ternary complex with GTP that interacts with the 40S subunit. The resulting complex then binds to an mRNA and scans for the AUG start codon. Eukaryotic translation initiation factor 5B (EIF5B) plays a role in recognition of the AUG codon in conjunction with translation factor eIF2, which functions to general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes, and promotes joining of the 60S ribosomal subunit. A single crossreactive polypeptide of 175 kDa was detected, whereas the predicted size of the protein was 139 kDa. This size discrepancy may be the result of posttranslational modifications of EIF5B or, perhaps more likely, of unusual behavior in SDS-PAGE caused by the highly charged N-terminal region of EIF5B (PMID: 10200264 ).