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验证数据展示

  • IF Staining of HEK-293 using CL488-27642

    IF Staining of HEK-293 using CL488-27642

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 ELA2 antibody (CL488-27642) at dilution of 1:100.

CoraLite® Plus 488-conjugated Neutrophil Elastase Polyclonal antibody

Neutrophil Elastase Polyclonal Antibody for IF/ICC
Cat No. CL488-27642

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

ELANE, ELA2, HNE, HLE, Elastase 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ELANE/ELA2 Ready-To-Use IHC Kit. ELANE/ELA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27642-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27642 targets Neutrophil Elastase in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Neutrophil Elastase fusion protein Ag26634 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name elastase 2, neutrophil
Synonyms ELANE, ELA2, HNE, HLE, Elastase 2
Calculated Molecular Weight 267 aa, 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC074816
Gene Symbol ELA2
Gene ID (NCBI) 1991
RRIDAB_3084110
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP08246
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELA2(Neutrophil elastase) is also named as ELAL, NE, HLE, HNE, PMN-E, SERP1, GE, ELANE and belongs to the peptidase S1 family. It is a 33-kDa enzyme with several isoforms that differ in their extent of glycosylation(PMID:12223222). Human neutrophil elastase (HNE) is a serine protease with potent proteolytic activity (3), which is reported to cause mucin secretion (degranulation)(PMID:12169572). It may contribute to the directed migration of leukocytes into flamed postcapillary venules in addition to contributing to the process of tissue emigration(PMID:9683424). The full length protein has a signal peptide,propeptide and three glycosylation sites.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Neutrophil Elastase antibody CL488-27642Download protocol
Standard Protocols
Click here to view our Standard Protocols