验证数据展示
经过测试的应用
Positive WB detected in | DU 145 cells, HeLa cells, HepG2 cells |
Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10071-1-AP targets ELAC2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rabbit |
免疫原 | ELAC2 fusion protein Ag0105 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | elaC homolog 2 (E. coli) |
别名 | elaC homolog 2 (E. coli), ElaC homolog protein 2, ELAC2, ELC2, HPC2, Ribonuclease Z 2, RNase Z 2, tRNA 3 endonuclease 2, tRNase Z 2 |
计算分子量 | 92 kDa |
观测分子量 | 92 kDa |
GenBank蛋白编号 | BC004158 |
基因名称 | ELAC2 |
Gene ID (NCBI) | 60528 |
RRID | AB_2096551 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BQ52 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).
实验方案
Product Specific Protocols | |
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WB protocol for ELAC2 antibody 10071-1-AP | Download protocol |
IHC protocol for ELAC2 antibody 10071-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cell Metab Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids. | ||
Nat Commun Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria. | ||
RNA Biol Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs.
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