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验证数据展示

  • WB analysis of DU 145 using 10071-1-AP

    WB analysis of DU 145 using 10071-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 10071-1-AP (ELAC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 10071-1-AP

    IHC staining of mouse testis using 10071-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10071-1-AP (ELAC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ELAC2 antibody included
  • Featured Product
  • KD/KO Validated

ELAC2 Polyclonal antibody

ELAC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10071-1-AP

Print datasheet

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA and More (1)

Synonyms

elaC homolog 2 (E. coli), ElaC homolog protein 2, ELAC2, ELC2, HPC2, Ribonuclease Z 2, RNase Z 2, tRNA 3 endonuclease 2, tRNase Z 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, HeLa cells, HepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ELAC2 Ready-To-Use IHC Kit. ELAC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10071-1-AP targets ELAC2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rabbit
Immunogen ELAC2 fusion protein Ag0105 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name elaC homolog 2 (E. coli)
Synonyms elaC homolog 2 (E. coli), ElaC homolog protein 2, ELAC2, ELC2, HPC2, Ribonuclease Z 2, RNase Z 2, tRNA 3 endonuclease 2, tRNase Z 2
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberBC004158
Gene Symbol ELAC2
Gene ID (NCBI) 60528
RRIDAB_2096551
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQ52
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

实验方案

Product Specific Protocols
WB protocol for ELAC2 antibody 10071-1-APDownload protocol
IHC protocol for ELAC2 antibody 10071-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Adv

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Authors - Kara L Perks
View Article
humanIF

Cell Metab

GRSF1 regulates RNA processing in mitochondrial RNA granules.

Authors - Jourdain Alexis A AA
View Article
humanWB

Cell Metab

Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.

Authors - Daniel F Bogenhagen
View Article
humanWB

Nat Commun

Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria.

Authors - Zbigniew Pietras
View Article
mouseWB

Cell Rep

Hierarchical RNA Processing Is Required for Mitochondrial Ribosome Assembly.

Authors - Oliver Rackham
View Article
humanWB,IF

RNA Biol

Involvement of human ELAC2 gene product in 3' end processing of mitochondrial tRNAs.

Authors - Brzezniak Lien K LK
  • KD Validated
View Article