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ELAC2 Recombinant antibody

ELAC2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240487B6

Cat no : 83468-6-RR

Print datasheet

Synonyms

FLJ10530, ELC2, ElaC homolog protein 2, elaC homolog 2 (E. coli), EC:3.1.26.11

验证数据展示

  • WB analysis using 83468-6-RR

    WB analysis using 83468-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83468-6-RR (ELAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A431 using 83468-6-RR

    FC experiment of A431 using 83468-6-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ELAC2 Recombinant antibody (83468-6-RR, Clone:240487B6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83468-6-RR

    Affinity and Kinetic Characterization of 83468-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83468-6-RR against Human ELAC2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Raji cells, DU 145 cells
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ELAC2 Ready-To-Use IHC Kit. ELAC2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83468-6-RR targets ELAC2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen ELAC2 fusion protein Ag0105 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name elaC homolog 2 (E. coli)
Synonyms FLJ10530, ELC2, ElaC homolog protein 2, elaC homolog 2 (E. coli), EC:3.1.26.11
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 97 kDa
GenBank Accession NumberBC004158
Gene Symbol ELAC2
Gene ID (NCBI) 60528
RRIDAB_3671098
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BQ52
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

实验方案

Product Specific Protocols
WB protocol for ELAC2 antibody 83468-6-RRDownload protocol
FC protocol for ELAC2 antibody 83468-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols