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ELAVL4 Monoclonal antibody, PBS Only

ELAVL4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1F5

Cat no : 67835-1-PBS

Print datasheet

Synonyms

HUD, Hu-antigen D, HuA/B/C/D, Hu C/D, ELAV-like protein 4

验证数据展示

  • WB analysis of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

    WB analysis of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • WB analysis of SH-SY5Y using 67835-1-Ig (same clone as 67835-1-PBS)

    WB analysis of SH-SY5Y using 67835-1-Ig (same clone as 67835-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • WB analysis using 67835-1-Ig (same clone as 67835-1-PBS)

    WB analysis using 67835-1-Ig (same clone as 67835-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67835-1-Ig (same clone as 67835-1-PBS)

    IHC staining of mouse brain using 67835-1-Ig (same clone as 67835-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 67835-1-Ig (same clone as 67835-1-PBS)

    IHC staining of mouse brain using 67835-1-Ig (same clone as 67835-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

    FC experiment of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ELAVL4 (67835-1-Ig, Clone:1B1F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

    IF Staining of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ELAVL4 antibody (67835-1-Ig, Clone: 1B1F5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

产品信息

67835-1-PBS targets ELAVL4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen ELAVL4 fusion protein Ag3678 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
Synonyms HUD, Hu-antigen D, HuA/B/C/D, Hu C/D, ELAV-like protein 4
Calculated Molecular Weight 380 aa, 42 kDa
Observed Molecular Weight32-40 kDa
GenBank Accession NumberBC036071
Gene Symbol ELAVL4
Gene ID (NCBI) 1996
RRIDAB_2918597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP26378
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )