ELAVL4 Monoclonal antibody
ELAVL4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, pig, rabbit
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B1F5
验证数据展示
经过测试的应用
| Positive WB detected in | pig brain tissue, U2OS cells, SH-SY5Y cells, rat brain tissue, mouse brain tissue, rabbit brain tissue, rat cerebellum tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | U2OS cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| CoIP | See 1 publications below |
产品信息
67835-1-Ig targets ELAVL4 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| Cited Applications | CoIP |
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Cited Reactivity | mouse |
| Immunogen | ELAVL4 fusion protein Ag3678 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) |
| Synonyms | HUD, Hu-antigen D, HuA/B/C/D, Hu C/D, ELAV-like protein 4 |
| Calculated Molecular Weight | 380 aa, 42 kDa |
| Observed Molecular Weight | 32-40 kDa |
| GenBank Accession Number | BC036071 |
| Gene Symbol | ELAVL4 |
| Gene ID (NCBI) | 1996 |
| RRID | AB_2918597 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26378 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ELAVL4 antibody 67835-1-Ig | Download protocol |
| IHC protocol for ELAVL4 antibody 67835-1-Ig | Download protocol |
| IF protocol for ELAVL4 antibody 67835-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
