验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse brain tissue |
| Positive IF/ICC detected in | U2OS cells |
| Positive FC (Intra) detected in | U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67835 targets ELAVL4 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| 经测试应用 | IF/ICC, IF-P, FC (Intra) Application Description |
| 经测试反应性 | human, mouse, rat, pig, rabbit |
| 免疫原 | ELAVL4 fusion protein Ag3678 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) |
| 别名 | HUD, Hu-antigen D, HuA/B/C/D, Hu C/D, ELAV-like protein 4 |
| 计算分子量 | 380 aa, 42 kDa |
| 观测分子量 | 32-40 kDa |
| GenBank蛋白编号 | BC036071 |
| 基因名称 | ELAVL4 |
| Gene ID (NCBI) | 1996 |
| RRID | AB_2923797 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P26378 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 ELAVL4 antibody CL488-67835 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |