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验证数据展示

  • WB analysis of HeLa using 26599-1-AP

    WB analysis of HeLa using 26599-1-AP

    WB result of ELOVL5 antibody (26599-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELOVL5 transfected HeLa cells.

  • IHC staining of mouse liver using 26599-1-AP

    IHC staining of mouse liver using 26599-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26599-1-AP (ELOVL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 26599-1-AP

    WB analysis using 26599-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26599-1-AP (ELOVL5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 26599-1-AP

    IF Staining of HeLa using 26599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ELOVL5 antibody (26599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of HepG2 using 26599-1-AP

    IF Staining of HepG2 using 26599-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ELOVL5 antibody (26599-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

ELOVL5 Polyclonal antibody

ELOVL5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26599-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

ELOVL2, ELOVL5, Fatty acid elongase 5, HELO1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26599-1-AP targets ELOVL5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ELOVL5 fusion protein Ag24786 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast)
别名 ELOVL2, ELOVL5, Fatty acid elongase 5, HELO1
计算分子量 299 aa, 35 kDa
观测分子量25 kDa
GenBank蛋白编号BC017270
基因名称 ELOVL5
Gene ID (NCBI) 60481
RRIDAB_3669551
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYP7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Very long-chain fatty acid elongase 5 (ELOVL5) is a key enzyme for the endogenous synthesis of long-chain unsaturated fatty acids. A critical role of ELOVL5 in elongating fatty acid substrates ranging from 16 to 20 carbons was reported in humans and rodents (PMID: 29454701). ELOVL5 is involved in metastasis through lipid droplets-regulated TGF-β receptor expression and is a predictive biomarker of metastatic ER+ breast cancer (PMID: 36056008). Western blot analysis detected ELOVL51 at an apparent molecular mass of ~25 kDa (PMID: 36056008, 32527375).

实验方案

Product Specific Protocols
WB protocol for ELOVL5 antibody 26599-1-APDownload protocol
IHC protocol for ELOVL5 antibody 26599-1-APDownload protocol
IF protocol for ELOVL5 antibody 26599-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols