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  • 概述
  • 经过测试的应用
  • 产品信息

EMB Polyclonal antibody, PBS Only

EMB Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13946-1-PBS

Print datasheet

Synonyms

验证数据展示

  • IHC staining of mouse testis using 13946-1-AP (same clone as 13946-1-PBS)

    IHC staining of mouse testis using 13946-1-AP (same clone as 13946-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13946-1-AP (EMB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 13946-1-AP (same clone as 13946-1-PBS)

    WB analysis of HeLa using 13946-1-AP (same clone as 13946-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13946-1-AP (EMB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13946-1-PBS in a different storage buffer formulation.

产品信息

13946-1-PBS targets EMB in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen EMB fusion protein Ag5031 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name embigin homolog (mouse)
Synonyms
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC059398
Gene Symbol EMB
Gene ID (NCBI) 133418
RRIDAB_2877993
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PCB8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycolyated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)