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Emerin Recombinant antibody, PBS Only

Emerin Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230089B1

Cat no : 82888-1-PBS

Print datasheet

Synonyms

EMD, STA, LEMD5, EDMD, 230089B1

验证数据展示

  • IF Staining of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    IF Staining of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Emerin antibody (82888-1-RR, Clone: 230089B1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    FC experiment of HeLa using 82888-1-RR (same clone as 82888-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Emerin Recombinant antibody (82888-1-RR, Clone:230089B1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 82888-1-RR (same clone as 82888-1-PBS)

    IHC staining of human stomach cancer using 82888-1-RR (same clone as 82888-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-1-RR (Emerin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82888-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82888-1-RR

    Affinity and Kinetic Characterization of 82888-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82888-1-RR against Human Emerin were performed. The affinity constant is 5.36 nM.

产品信息

82888-1-PBS targets Emerin in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Emerin fusion protein Ag0236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name emerin
Synonyms EMD, STA, LEMD5, EDMD, 230089B1
Calculated Molecular Weight 34 kDa
GenBank Accession NumberBC000738
Gene Symbol EMD
Gene ID (NCBI) 2010
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50402
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.