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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 82888-3-RR (same clone as 82888-3-PBS)

    WB analysis using 82888-3-RR (same clone as 82888-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82888-3-RR (Emerin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

    WB analysis of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

    WB result of Emerin antibody (82888-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Emerin transfected HeLa cells. This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

    FC experiment of HeLa using 82888-3-RR (same clone as 82888-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug EMD Recombinant antibody (82888-3-RR, Clone:230089B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 82888-3-RR (same clone as 82888-3-PBS)

    IHC staining of human stomach cancer using 82888-3-RR (same clone as 82888-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-3-RR (Emerin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9). This data was developed using the same antibody clone with 82888-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82888-3-RR

    Affinity and Kinetic Characterization of 82888-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82888-3-RR against Human EMD were performed. The affinity constant is 11.1 nM.

  • Featured Product
  • KD/KO Validated

Emerin Recombinant antibody, PBS Only

Emerin Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA
Cat No. 82888-3-PBS

Print datasheet

CloneNo. 230089B12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

EMD, STA, LEMD5, EDMD, 230089B12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

82888-3-PBS targets Emerin in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen Emerin fusion protein Ag0236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name emerin
Synonyms EMD, STA, LEMD5, EDMD, 230089B12
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC000738
Gene Symbol EMD
Gene ID (NCBI) 2010
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP50402
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C