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验证数据展示

  • WB analysis using 82888-3-RR

    WB analysis using 82888-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82888-3-RR (Emerin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 82888-3-RR

    WB analysis of HeLa using 82888-3-RR

    WB result of Emerin antibody (82888-3-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Emerin transfected HeLa cells.

  • FC experiment of HeLa using 82888-3-RR

    FC experiment of HeLa using 82888-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug EMD Recombinant antibody (82888-3-RR, Clone:230089B12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IHC staining of human stomach cancer using 82888-3-RR

    IHC staining of human stomach cancer using 82888-3-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 82888-3-RR (Emerin antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Affinity and Kinetic Characterization of 82888-3-RR

    Affinity and Kinetic Characterization of 82888-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82888-3-RR against Human EMD were performed. The affinity constant is 11.1 nM.

  • Featured Product
  • KD/KO验证

Emerin Recombinant antibody

Emerin Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82888-3-RR

产品说明书

CloneNo. 230089B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

EMD, STA, LEMD5, EDMD, 230089B12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82888-3-RR targets Emerin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Emerin fusion protein Ag0236 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 emerin
别名 EMD, STA, LEMD5, EDMD, 230089B12
计算分子量 34 kDa
观测分子量 35 kDa
GenBank蛋白编号BC000738
基因名称 EMD
Gene ID (NCBI) 2010
RRIDAB_3670610
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP50402
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Emerin antibody 82888-3-RRDownload protocol
IHC protocol for Emerin antibody 82888-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols