验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive FC (Intra) detected in | HEK-293T cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-82888-3 targets Emerin in WB, FC (Intra) applications and shows reactivity with human samples.
| Tested Applications | WB, FC (Intra) Application Description |
| Tested Reactivity | human |
| Immunogen | Emerin fusion protein Ag0236 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | emerin |
| Synonyms | EMD, STA, LEMD5, EDMD, 230089B12 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC000738 |
| Gene Symbol | EMD |
| Gene ID (NCBI) | 2010 |
| RRID | AB_3673743 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P50402 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 Emerin antibody CL750-82888-3 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |