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验证数据展示

  • WB analysis using CL750-82888-3

    WB analysis using CL750-82888-3

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82888-3 (Emerin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293T using CL750-82888-3

    FC experiment of HEK-293T using CL750-82888-3

    1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated Emerin Recombinant antibody (CL750-82888-3, Clone:230089B12)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 750-conjugated Emerin Recombinant antibody

Emerin Uni-rAbTM Recombinant Antibody for WB, FC (Intra)
Cat No. CL750-82888-3

产品说明书

CloneNo. 230089B12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra)

别名

EMD, STA, LEMD5, EDMD, 230089B12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive FC (Intra) detected inHEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 82888-3-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82888-3 targets Emerin in WB, FC (Intra) applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra) Application Description
经测试反应性 human
免疫原 Emerin fusion protein Ag0236 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 emerin
别名 EMD, STA, LEMD5, EDMD, 230089B12
计算分子量 34 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC000738
基因名称 EMD
Gene ID (NCBI) 2010
RRIDAB_3673743
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP50402
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Emerin antibody CL750-82888-3Download protocol
Standard Protocols
Click here to view our Standard Protocols