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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated Emerin Recombinant antibody

Emerin Uni-rAbTM Recombinant Antibody for WB, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230089B12

Cat no : CL750-82888-3

Print datasheet

Synonyms

EMD, STA, LEMD5, EDMD, 230089B12

验证数据展示

  • WB analysis using CL750-82888-3

    WB analysis using CL750-82888-3

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82888-3 (Emerin antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • FC experiment of HEK-293T using CL750-82888-3

    FC experiment of HEK-293T using CL750-82888-3

    1x10^6 HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated Emerin Recombinant antibody (CL750-82888-3, Clone:230089B12)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells
Positive FC (Intra) detected inHEK-293T cells
For other applications, we recommend the unconjugated version of this antibody, 82888-3-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-82888-3 targets Emerin in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human
Immunogen Emerin fusion protein Ag0236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name emerin
Synonyms EMD, STA, LEMD5, EDMD, 230089B12
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC000738
Gene Symbol EMD
Gene ID (NCBI) 2010
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP50402
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Emerin antibody CL750-82888-3Download protocol
Standard Protocols
Click here to view our Standard Protocols