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验证数据展示

  • WB analysis of human kidney using 10643-1-AP

    WB analysis of human kidney using 10643-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 10643-1-AP (EMILIN1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 10643-1-AP

    IHC staining of human ovary tumor using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10643-1-AP (EMILIN1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with EMILIN1 antibody included
  • IHC staining of human colon using 10643-1-AP

    IHC staining of human colon using 10643-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10643-1-AP (EMILIN1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EMILIN1 antibody included
  • IF Staining of SKOV-3 using 10643-1-AP

    IF Staining of SKOV-3 using 10643-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using EMILIN1 antibody (10643-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).

EMILIN1 Polyclonal antibody

EMILIN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 10643-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Elastin microfibril interface-located protein 1, EMI, EMILIN, EMILIN 1, EMILIN-1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue, human brain tissue, human placenta tissue
Positive IHC detected inhuman colon tissue, human heart tissue, human kidney tissue, human ovary tissue, human ovary tumor tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy EMILIN1 Ready-To-Use IHC Kit. EMILIN1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10643-1-AP targets EMILIN1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 EMILIN1 fusion protein Ag1016 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 elastin microfibril interfacer 1
别名 Elastin microfibril interface-located protein 1, EMI, EMILIN, EMILIN 1, EMILIN-1
计算分子量 107 kDa
观测分子量 120 kDa
GenBank蛋白编号BC007530
基因名称 EMILIN1
Gene ID (NCBI) 11117
RRIDAB_2100192
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6C2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EMILIN1, also named EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).

实验方案

Product Specific Protocols
WB protocol for EMILIN1 antibody 10643-1-APDownload protocol
IHC protocol for EMILIN1 antibody 10643-1-APDownload protocol
IF protocol for EMILIN1 antibody 10643-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Res

Comparative oncogenomics identifies PSMB4 and SHMT2 as potential cancer driver genes.

Authors - Genee Y Lee
View Article