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EMILIN1 Monoclonal antibody, PBS Only

EMILIN1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5C8

Cat No : 60047-1-PBS

Print datasheet

Synonyms

EMI, EMILIN, EMILIN 1, EMILIN1, gp115

验证数据展示

  • WB analysis of human colon using 60047-1-Ig (same clone as 60047-1-PBS)

    WB analysis of human colon using 60047-1-Ig (same clone as 60047-1-PBS)

    Untreated and PNGase F-treated lysates of human colon tissue were subjected to SDS PAGE followed by western blot with 60047-1-Ig (EMILIN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EMILIN1 antibody included
  • IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    IHC staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60047-1-Ig (EMILIN1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EMILIN1 antibody included
  • IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

  • IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    IF Staining of human ovary tumor using 60047-1-Ig (same clone as 60047-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using EMILIN1 antibody (60047-1-Ig, Clone: 2A5C8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60047-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EMILIN1 Ready-To-Use IHC Kit. EMILIN1 primary antibody included.

产品信息

60047-1-PBS targets EMILIN1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen EMILIN1 fusion protein Ag1016 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name elastin microfibril interfacer 1
Synonyms EMI, EMILIN, EMILIN 1, EMILIN1, gp115
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC007530
Gene Symbol EMILIN1
Gene ID (NCBI) 11117
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y6C2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).