验证数据展示
经过测试的应用
| Positive WB detected in | human colon tissue, human rectum tissue |
| Positive IHC detected in | human ovary tumor tissue, human lung cancer tissue, human kidney tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human ovary tumor tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
60047-1-Ig targets EMILIN1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | EMILIN1 fusion protein Ag1016 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | elastin microfibril interfacer 1 |
| 别名 | EMI, EMILIN, EMILIN 1, EMILIN1, gp115 |
| 计算分子量 | 107 kDa |
| 观测分子量 | 120-130 kDa |
| GenBank蛋白编号 | BC007530 |
| 基因名称 | EMILIN1 |
| Gene ID (NCBI) | 11117 |
| RRID | AB_2100191 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9Y6C2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for EMILIN1 antibody 60047-1-Ig | Download protocol |
| IHC protocol for EMILIN1 antibody 60047-1-Ig | Download protocol |
| IF protocol for EMILIN1 antibody 60047-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Connect Tissue Res EMILIN-1 regulates the amount of oxytalan fiber formation in periodontal ligaments in vitro. | ||
