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CoraLite® Plus 488-conjugated EMILIN1 Monoclonal antibody

EMILIN1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A5C8

Cat no : CL488-60047

Print datasheet

Synonyms

DKFZp586M121, EMI, EMILIN, EMILIN 1, EMILIN1, gp115

验证数据展示

  • IF Staining of human ovary tumor using CL488-60047

    IF Staining of human ovary tumor using CL488-60047

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 EMILIN1 antibody (CL488-60047, Clone: 2A5C8 ) at dilution of 1:200.

  • IF Staining of human ovary tumor using CL488-60047

    IF Staining of human ovary tumor using CL488-60047

    Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using CoraLite® Plus 488 EMILIN1 antibody (CL488-60047, Clone: 2A5C8 ) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inhuman ovary tumor tissue
Planning an IHC experiment? We recommend our IHCeasy EMILIN1 Ready-To-Use IHC Kit. EMILIN1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60047-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60047 targets EMILIN1 in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen EMILIN1 fusion protein Ag1016 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name elastin microfibril interfacer 1
Synonyms DKFZp586M121, EMI, EMILIN, EMILIN 1, EMILIN1, gp115
Calculated Molecular Weight 107 kDa
Observed Molecular Weight 120-130 kDa
GenBank Accession NumberBC007530
Gene Symbol EMILIN1
Gene ID (NCBI) 11117
RRIDAB_2919227
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9Y6C2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EMILIN1, also named as EMILIN, EMILIN-1, gp115 and EMI, may be responsible for anchoring smooth muscle cells to elastic fibers, and may be involved not only in the formation of the elastic fiber, but also in the processes that regulate vessel assembly. It has cell adhesive capacity. EMILIN1 is a negative regulator of the transforming growth factor-beta (TGF-beta) signaling, which is involved in blood pressure (BP) homeostasis (PMID:20186130). EMILIN1 may play a key role in hypertensive vascular remodeling. TGFβ proteins are the main regulators of blood vessel development and maintenance, and EMILIN1 inhibits TGFβ signaling by binding specifically to the proTGFβ precursor and preventing its maturation by furin convertases in the extracellular space (PMID:19922630).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EMILIN1 antibody CL488-60047Download protocol
Standard Protocols
Click here to view our Standard Protocols