CoraLite® Plus 488-conjugated ENO1 Monoclonal antibody
ENO1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgM
Reactivity
Human, Mouse, Rat, Pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1D6E8
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67187-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-67187 targets ENO1 in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human, Mouse, Rat, Pig |
Immunogen | ENO1 fusion protein Ag17518 种属同源性预测 |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Full Name | enolase 1, (alpha) |
Synonyms | Alpha enolase, C myc promoter binding protein, ENO1, ENO1L1, Enolase 1, enolase 1, (alpha), MBP 1, MBPB1, MPB 1, MPB1, NNE, Non neural enolase, Phosphopyruvate hydratase, Plasminogen binding protein, PPH |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 47 kDa |
GenBank Accession Number | BC015641 |
Gene Symbol | ENO1 |
Gene ID (NCBI) | 2023 |
ENSEMBL Gene ID | ENSG00000074800 |
RRID | AB_2919432 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Thiophilic affinity chromatograph |
UNIPROT ID | P06733 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656)
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 ENO1 antibody CL488-67187 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |