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  • Featured Product
  • KD/KO Validated

Cardinal Red™-conjugated ENO1 Polyclonal antibody

ENO1 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

Cardinal Red™ Fluorescent Dye

Cat no : CR-11204

Print datasheet

Synonyms

Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase

验证数据展示

  • FC experiment of HeLa using CR-11204

    FC experiment of HeLa using CR-11204

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human ENO1 (CR-11204) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ENO1 Ready-To-Use IHC Kit. ENO1 primary antibody included.

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CR-11204 targets ENO1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ENO1 fusion protein Ag1692 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enolase 1, (alpha)
Synonyms Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC015641
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
Conjugate Cardinal Red™ Fluorescent Dye
Excitation/Emission Maxima Wavelengths592 nm / 611 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06733
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enolase is an important glycolytic enzyme involved in the interconversion of 2-phosphoglycerate to phosphoenolpyruvate.Enolase were down-regulated in oxLDL-treated cells or in VLDL-treated cells .And it identified which are associated with glucose metabolism were down-regulated in the process of foam cells formation.(19756395)

实验方案

Product Specific Protocols
FC protocol for Cardinal Red™ ENO1 antibody CR-11204Download protocol
Standard Protocols
Click here to view our Standard Protocols