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ENO3 Polyclonal antibody

ENO3 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16421-1-AP

Print datasheet

Synonyms

Muscle-specific enolase, MSE, GSD13, EC:4.2.1.11, Beta-enolase

验证数据展示

  • IF Staining of HeLa using 16421-1-AP

    IF Staining of HeLa using 16421-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16421-1-AP (ENO3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of human skeletal muscle using 16421-1-AP

    WB analysis of human skeletal muscle using 16421-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman skeletal muscle tissue, human brain tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ENO3 Ready-To-Use IHC Kit. ENO3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

16421-1-AP targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen ENO3 fusion protein Ag9613 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enolase 3 (beta, muscle)
Synonyms Muscle-specific enolase, MSE, GSD13, EC:4.2.1.11, Beta-enolase
Calculated Molecular Weight 434 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC017249
Gene Symbol ENO3
Gene ID (NCBI) 2027
RRIDAB_2099464
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13929
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13).

实验方案

Product Specific Protocols
WB protocol for ENO3 antibody 16421-1-APDownload protocol
IF protocol for ENO3 antibody 16421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Mol Cell Res

Phospholipase A2-activating protein induces mitophagy trough anti-apoptotic MCL1-mediated NLRX1 oligomerization

Authors - Zhilong Zheng
View Article
mouseWB

Mol Cell Proteomics

Extended Multiplexing of Tandem Mass Tags (TMT) Labeling Reveals Age and High Fat Diet Specific Proteome Changes in Mouse Epididymal Adipose Tissue.

Authors - Deanna L Plubell
View Article
ratWB

Cell Physiol Biochem

Gender-dimorphic regulation of skeletal muscle proteins in streptozotocin-induced diabetic rats.

Authors - Choi Minji M
View Article