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ENOPH1 Polyclonal antibody

ENOPH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17731-1-AP

Print datasheet

Synonyms

MASA, MST145, Enolase-phosphatase E1, EC:3.1.3.77, E1

验证数据展示

  • WB analysis using 17731-1-AP

    WB analysis using 17731-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17731-1-AP (ENOPH1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 17731-1-AP

    IHC staining of human liver cancer using 17731-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17731-1-AP (ENOPH1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 17731-1-AP

    IHC staining of human liver cancer using 17731-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17731-1-AP (ENOPH1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 17731-1-AP

    IF Staining of U-251 using 17731-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using ENOPH1 antibody (17731-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHL-60 cells, K-562 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

17731-1-AP targets ENOPH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen ENOPH1 fusion protein Ag12131 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name enolase-phosphatase 1
Synonyms MASA, MST145, Enolase-phosphatase E1, EC:3.1.3.77, E1
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight 29-35 kDa
GenBank Accession NumberBC065815
Gene Symbol ENOPH1
Gene ID (NCBI) 58478
RRIDAB_2099471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHY7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENOPH1, also named as MASA and MSTP145, belongs to the HAD-like hydrolase superfamily and MasA/MtnC family. ENOPH1 is a newly identified enzyme involved in L-methionine biosynthesis, which is associated with anxiety and depression. Studies have shown that ENOPH1 plays a crucial role in promoting the proliferation and migration of glioma cells (PMID: 32654229). ENOPH1 has 2 isoforms with the molecular mass of 13 and 29 kDa.

实验方案

Product Specific Protocols
WB protocol for ENOPH1 antibody 17731-1-APDownload protocol
IHC protocol for ENOPH1 antibody 17731-1-APDownload protocol
IF protocol for ENOPH1 antibody 17731-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Exp Neurol

Evidence that enolase-phosphatase 1 exacerbates early cerebral ischemia injury and blood-brain barrier breakdown by enhancing extracellular matrix destruction and inhibiting the interaction between ADI1 and MT1-MMP

Authors - Dexin Yang
View Article