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  • Featured Product
  • KD/KO Validated

ENOX2 Polyclonal antibody

ENOX2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10423-1-AP

Print datasheet

Synonyms

Protein disulfide-thiol oxidoreductase, Hydroquinone [NADH] oxidase, Ecto-NOX disulfide-thiol exchanger 2, COVA1, APK1 antigen

验证数据展示

  • WB analysis of MCF-7 using 10423-1-AP

    WB analysis of MCF-7 using 10423-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 10423-1-AP (ENOX2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 10423-1-AP

    IHC staining of human ovary tumor using 10423-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10423-1-AP (ENOX2 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of MCF-7 using 10423-1-AP

    IP experiment of MCF-7 using 10423-1-AP

    IP result of anti-ENOX2 (IP:10423-1-AP, 3ug; Detection:10423-1-AP 1:700) with MCF-7 cells lysate 4000ug.

  • IF Staining of MCF-7 using 10423-1-AP

    IF Staining of MCF-7 using 10423-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using ENOX2 antibody (10423-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells, mouse ovary tissue, PC-3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10423-1-AP targets ENOX2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen ENOX2 fusion protein Ag0674 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ecto-NOX disulfide-thiol exchanger 2
Synonyms Protein disulfide-thiol oxidoreductase, Hydroquinone [NADH] oxidase, Ecto-NOX disulfide-thiol exchanger 2, COVA1, APK1 antigen
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC019254
Gene Symbol ENOX2
Gene ID (NCBI) 10495
RRIDAB_2099483
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16206
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock

实验方案

Product Specific Protocols
WB protocol for ENOX2 antibody 10423-1-APDownload protocol
IHC protocol for ENOX2 antibody 10423-1-APDownload protocol
IF protocol for ENOX2 antibody 10423-1-APDownload protocol
IP protocol for ENOX2 antibody 10423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Chem

Synthesis and characterization of 4,11-diaminoanthra[2,3-b]furan-5,10-diones: tumor cell apoptosis through tNOX-modulated NAD+/NADH ratio and SIRT1.

Authors - Alexander S Tikhomirov
View Article
humanIP

Am J Cancer Res

Capsaicin acts through tNOX (ENOX2) to induce autophagic apoptosis in p53-mutated HSC-3 cells but autophagy in p53-functional SAS oral cancer cells.

Authors - Chin-Fang Chang
View Article
human,mouseIP

Am J Cancer Res

Capsaicin exerts therapeutic effects by targeting tNOX-SIRT1 axis and augmenting ROS-dependent autophagy in melanoma cancer cells.

Authors - Atikul Islam
View Article
humanWB

Exp Cell Res

Phosphorylation of serine-504 of tNOX (ENOX2) modulates cell proliferation and migration in cancer cells.

Authors - Zeng Zih-Ming ZM
  • KD Validated
View Article
humanWB

J Agric Food Chem

Capsaicin-mediated tNOX (ENOX2) up-regulation enhances cell proliferation and migration in vitro and in vivo.

Authors - Liu Nei-Chi NC
View Article
humanWB

Biochem Biophys Res Commun

RNA interference targeting tNOX attenuates cell migration via a mechanism that involves membrane association of Rac.

Authors - Liu Shan-Chi SC
  • KD Validated
View Article