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验证数据展示

  • IF Staining of MCF-7 using CL488-10423

    IF Staining of MCF-7 using CL488-10423

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 ENOX2 antibody (CL488-10423) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-10423

    FC experiment of MCF-7 using CL488-10423

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated ENOX2 Polyclonal antibody (CL488-10423)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated ENOX2 Polyclonal antibody

ENOX2 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-10423

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

Protein disulfide-thiol oxidoreductase, Hydroquinone [NADH] oxidase, Ecto-NOX disulfide-thiol exchanger 2, COVA1, APK1 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 10423-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-10423 targets ENOX2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 ENOX2 fusion protein Ag0674 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ecto-NOX disulfide-thiol exchanger 2
别名 Protein disulfide-thiol oxidoreductase, Hydroquinone [NADH] oxidase, Ecto-NOX disulfide-thiol exchanger 2, COVA1, APK1 antigen
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC019254
基因名称 ENOX2
Gene ID (NCBI) 10495
RRIDAB_3672441
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16206
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Tumor-associated NADH oxidase (ENOX2) as a member of a family of growth-related NADH (or hydroquinone) oxidases. It functions as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 22 minutes and play a role in control of the ultradian cellular biological clock

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ENOX2 antibody CL488-10423Download protocol
FC protocol for CL Plus 488 ENOX2 antibody CL488-10423Download protocol
Standard Protocols
Click here to view our Standard Protocols