CD39/ENTPD1 Polyclonal antibody

CD39/ENTPD1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19229-1-AP

Print datasheet

Synonyms

ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1



经过测试的应用

Positive WB detected inmouse liver tissue, HL-60 cells, mouse brain tissue, human liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman placenta tissue, human liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19229-1-AP targets CD39/ENTPD1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen CD39/ENTPD1 fusion protein Ag6165 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ectonucleoside triphosphate diphosphohydrolase 1
Synonyms ATPDase, CD39, CD39/ENTPD1, Ecto apyrase, Ecto ATP diphosphohydrolase 1, Ecto ATPase 1, Ecto ATPDase 1, ENTPD1, e-NTPDase, NTPDase 1
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 75-95 kDa
GenBank Accession NumberBC047664
Gene Symbol CD39
Gene ID (NCBI) 953
RRIDAB_10859825
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49961
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ENTPD1(Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated(PMID:7930580). It has 3 isoforms produced by alternative splicing. CD39 preferentially occurred as a dimer that could be dissociated into monomeric forms. (PMID: 12694193). The soluble isoform of CD39(38-478 amino acid) is located in the extracellular part and 19229-1-AP recognizes both the 3 isoforms and the soluble form of CD39 (38-478aa) molecule.

实验方案

Product Specific Protocols
WB protocol for CD39/ENTPD1 antibody 19229-1-APDownload protocol
IHC protocol for CD39/ENTPD1 antibody 19229-1-APDownload protocol
IF protocol for CD39/ENTPD1 antibody 19229-1-APDownload protocol
IP protocol for CD39/ENTPD1 antibody 19229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Bone Res

Alpl prevents bone ageing sensitivity by specifically regulating senescence and differentiation in mesenchymal stem cells.

Authors - Wenjia Liu
humanWB

Biochim Biophys Acta Mol Basis Dis

Extracellular ATP (eATP) inhibits the progression of endometriosis and enhances the immune function of macrophages

Authors - Ling Zhou
humanFC

J Chem Inf Model

Identification of Novel CD39 Inhibitors Based on Virtual Screening and Enzymatic Assays.

Authors - Yunshuo Zhao
ratWB

Biochem J

Characterization of soluble CD39 (SolCD39/NTPDase1) from PiggyBac nonviral system as a tool to control the nucleotides level.

Authors - Liziane Raquel Beckenkamp
mouseIHC

JCI Insight

Epithelial microRNA-206 targets CD39/extracellular ATP to upregulate airway IL-25 and TSLP in type 2-high asthma.

Authors - Kan Zhang
mouseWB,IHC

Cancer Immunol Immunother

STAT3 promotes differentiation of monocytes to MDSCs via CD39/CD73-adenosine signal pathway in oral squamous cell carcinoma

Authors - Hao Cui