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验证数据展示

  • WB analysis using 84732-2-RR

    WB analysis using 84732-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84732-2-RR (EPB41L4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84732-2-RR

    Affinity and Kinetic Characterization of 84732-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84732-2-RR against Human EPB41L4B were performed. The affinity constant is 6.86 nM.

EPB41L4B Recombinant antibody

EPB41L4B Recombinant Antibody for WB, ELISA
Cat No. 84732-2-RR

产品说明书

CloneNo. 242186G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

FERM containing protein CG1, EHM2, CG1, Band 4.1 like protein 4B, 242186G4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293T cells, HepG2 cells, HaCaT cells, mouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84732-2-RR targets EPB41L4B in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 EPB41L4B fusion protein Ag36353 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 erythrocyte membrane protein band 4.1 like 4B
别名 FERM containing protein CG1, EHM2, CG1, Band 4.1 like protein 4B, 242186G4
计算分子量99kDa
观测分子量100 kDa
GenBank蛋白编号NM_00138562
基因名称 EPB41L4B
Gene ID (NCBI) 54566
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H329
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The 4.1 proteins, encoded by the EPB41 (erythrocyte protein band 4.1) genes, are components of the cortical cytoskeleton underlying the cell membrane. The family of 4.1 proteins consists of the eponymous 4.1R protein first identified in erythrocytes (gene: EPB41), 4.1N (EPB41L1), 4.1G (EPB41L2), 4.1B (EPB41L3) as well as the less closely related members NBL4 (EPB41L4A), EHM2 (EPB41L4B) and EPB41L5 (EPB41L5). EHM2 is conspicuous in tumor cells with high migratory potential, such as metastatic melanoma and fibrosarcoma cells. In prostate cancer, EHM2 has been reported to be overexpressed and to diminish adhesion of prostate cancer cells to collagen. (PMID: 20860828)

实验方案

Product Specific Protocols
WB protocol for EPB41L4B antibody 84732-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols