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验证数据展示

  • WB analysis of HeLa using 60155-2-Ig (same clone as 60155-2-PBS)

    WB analysis of HeLa using 60155-2-Ig (same clone as 60155-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60155-2-Ig (EPHA1-specific antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    IHC staining of human breast cancer using 60155-2-Ig (same clone as 60155-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60155-2-Ig (EPHA1-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60155-2-PBS in a different storage buffer formulation.

EPHA1-specific Monoclonal antibody, PBS Only

EPHA1-specific Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 60155-2-PBS

产品说明书

CloneNo. 1B7C3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

EPH, EPH receptor A1, EPHA1, EPHA1-specific, Ephrin type A receptor 1, EPHT, EPHT1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60155-2-PBS targets EPHA1-specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 EPH receptor A1
别名 EPH, EPH receptor A1, EPHA1, EPHA1-specific, Ephrin type A receptor 1, EPHT, EPHT1
计算分子量 108 kDa
观测分子量 108 kDa
GenBank蛋白编号BC130291
基因名称 EPHA1
Gene ID (NCBI) 2041
RRIDAB_10646478
偶联类型 Unconjugated
形式 Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP21709
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPHA1, also named as EPH, EPHT and EPHT1, belongs to ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. Ligands in the ephrin-A subclass, including the prototype family member ephrin-A1 (B61), are membrane associated through glycosylphosphatidyl-inositol linkages, whereas ephrin-B subclass consists of ligands with transmembrane domains. The general role of the Eph family is in mediating repulsive cell-cell interaction.