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  • Featured Product
  • KD/KO Validated

EPHA2 Monoclonal antibody, PBS Only

EPHA2 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A9C3

Cat no : 66736-1-PBS

Print datasheet

Synonyms

Ephrin type-A receptor 2, Ephrin type A receptor 2, EPH receptor A2, ECK, EC:2.7.10.1

验证数据展示

  • IF Staining of HepG2 using 66736-1-Ig (same clone as 66736-1-PBS)

    IF Staining of HepG2 using 66736-1-Ig (same clone as 66736-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EPHA2 antibody (66736-1-Ig, Clone: 1A9C3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

  • WB analysis using 66736-1-Ig (same clone as 66736-1-PBS)

    WB analysis using 66736-1-Ig (same clone as 66736-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66736-1-Ig (EPHA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66736-1-PBS in a different storage buffer formulation.

产品信息

66736-1-PBS targets EPHA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen EPHA2 fusion protein Ag22566 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name EPH receptor A2
Synonyms Ephrin type-A receptor 2, Ephrin type A receptor 2, EPH receptor A2, ECK, EC:2.7.10.1
Calculated Molecular Weight 976 aa, 108 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC037166
Gene Symbol EPHA2
Gene ID (NCBI) 1969
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29317
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EPHA2 (Ephrin type-A receptor 2) belongs to the receptor tyrosine kinase (RTK) family, with 16 known receptors and 9 known membrane-bound ligands in all species. Based on the extracellular domain sequence homology, structure, and binding affinity, Eph receptors and their ephrin ligands can be divided into A and B subtypes (PMID: 33962882). EPHA2 contains a conserved N-terminal ligand-bound extracellular domain, a transmembrane domain, and a conserved tyrosine kinase domain (PMID: 8070404).