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验证数据展示

  • WB analysis using 66982-1-Ig

    WB analysis using 66982-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66982-1-Ig (EPHX1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 66982-1-Ig

    WB analysis of HeLa using 66982-1-Ig

    WB result of EPHX1 antibody (66982-1-Ig; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPHX1 transfected HeLa cells.

  • Featured Product
  • KD/KO Validated

EPHX1 Monoclonal antibody

EPHX1 Monoclonal Antibody for WB, ELISA
Cat No. 66982-1-Ig

Print datasheet

CloneNo. 1B6A2

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse

Applications

WB, ELISA

Synonyms

EPHX, EPHX1, EPOX, Epoxide hydratase, Epoxide hydrolase 1, MEH, Microsomal epoxide hydrolase

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, 4T1 cells, HepG2 cells, L02 cells, NCI-H1299 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66982-1-Ig targets EPHX1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen EPHX1 fusion protein Ag25820 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name epoxide hydrolase 1, microsomal (xenobiotic)
Synonyms EPHX, EPHX1, EPOX, Epoxide hydratase, Epoxide hydrolase 1, MEH, Microsomal epoxide hydrolase
Calculated Molecular Weight 53 kDa
Observed Molecular Weight53 kDa
GenBank Accession NumberBC003567
Gene Symbol EPHX1
Gene ID (NCBI) 2052
RRIDAB_3085034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07099
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPHX1, also known as EPHX and EPOX, belongs to the peptidase S33 family. EPHX1 is an evolutionarily highly conserved biotransformation enzyme expressed in nearly all tissues and localized mainly in the microsomal fraction of the endoplasmic reticulum of eukaryotic cells. EPHX1 plays a role in the metabolism of endogenous lipids such as epoxide-containing fatty acids. The calculated molecular weight of EPHX1 is 53 kDa (PMID: 26216302).

实验方案

Product Specific Protocols
WB protocol for EPHX1 antibody 66982-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols