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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated EPLIN Monoclonal antibody

EPLIN Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

6E6E6

Cat no : CL594-66071

Print datasheet

Synonyms

EPLIN, FLJ38853, LIM domain and actin binding 1, LIMA1, SREBP3

验证数据展示

  • IF Staining of HeLa using CL594-66071

    IF Staining of HeLa using CL594-66071

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66071 (EPLIN antibody) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66071-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL594-66071 is EPLIN Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human, rat
Immunogen EPLIN fusion protein Ag13288 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name LIM domain and actin binding 1
Synonyms EPLIN, FLJ38853, LIM domain and actin binding 1, LIMA1, SREBP3
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 90 kDa, 110 kDa
GenBank Accession NumberBC001247
Gene Symbol EPLIN
Gene ID (NCBI) 51474
RRIDAB_2883502
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UHB6
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

实验方案

Product Specific Protocols
IF protocol for CL594 EPLIN antibody CL594-66071Download protocol
Standard Protocols
Click here to view our Standard Protocols